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  • EI
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中國精品科技期刊2020
朱雨馨,王文斌,王佳婧,等. 富鍺木耳菌絲體不同溶劑提取物的體外抗氧化能力[J]. 食品工業科技,2024,45(8):21?28. doi: 10.13386/j.issn1002-0306.2023070197.
引用本文: 朱雨馨,王文斌,王佳婧,等. 富鍺木耳菌絲體不同溶劑提取物的體外抗氧化能力[J]. 食品工業科技,2024,45(8):21?28. doi: 10.13386/j.issn1002-0306.2023070197.
ZHU Yuxin, WANG Wenbin, WANG Jiajing, et al. Antioxidant Capacity of Different Solvent Extracts from Ge-riched Auricularia auricula Mycelium in Vitro[J]. Science and Technology of Food Industry, 2024, 45(8): 21?28. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070197.
Citation: ZHU Yuxin, WANG Wenbin, WANG Jiajing, et al. Antioxidant Capacity of Different Solvent Extracts from Ge-riched Auricularia auricula Mycelium in Vitro[J]. Science and Technology of Food Industry, 2024, 45(8): 21?28. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070197.

富鍺木耳菌絲體不同溶劑提取物的體外抗氧化能力

Antioxidant Capacity of Different Solvent Extracts from Ge-riched Auricularia auricula Mycelium in Vitro

  • 摘要: 為探索富鍺木耳菌絲體不同溶劑提取物的體外抗氧化能力,以富鍺木耳菌絲體為材料,通過水、70%乙醇和乙酸乙酯對富鍺木耳菌絲體進行了物質提取,比較了不同提取物對超氧陰離子自由基、羥自由基和DPPH自由基的清除能力。采用隸屬函數值評價了不同溶劑提取物的綜合抗氧化能力,并對物質含量與抗氧化能力進行了相關性分析。結果表明:富鍺木耳菌絲體有機鍺含量為542.83 μg/g。不同溶劑提取物的提取率和物質含量均不同,水和乙醇提取物的提取率較高,水提取物中多糖含量最多,乙酸乙酯提取物中多酚和黃酮含量最多。不同溶劑提取物對自由基均有一定的清除能力,且隨著提取物濃度的增加而增加。水提取物對超氧陰離子自由基清除率最強,當提取物濃度為9 mg/mL時,清除率最高為43.32%。70%乙醇提取物對羥自由基和DPPH自由基的清除效果最好,當提取物濃度為9 mg/mL時,清除率分別為70.10%和76.99%。多糖、多酚和黃酮在清除自由基中均發揮不同的作用,70%乙醇提取物的綜合抗氧化能力最強。多糖、多酚和黃酮含量均與DPPH自由基清除率有顯著(P<0.05)正相關。70%乙醇提取物中多酚含量與羥自由基清除率有顯著(P<0.05)正相關。多糖、多酚及黃酮對超氧陰離子自由基的清除率均有顯著(P<0.05)影響。富鍺木耳菌絲體含有較高含量的有機鍺,提取物對自由基具有一定的清除能力,具有較好的抗氧化性能。本研究對開發富鍺木耳功能食品具有一定指導意義。

     

    Abstract: In order to explore the antioxidant ability of different solvent extracts of Ge-riched Auricularia auricular mycelium in vitro, the Ge-riched A. auricular mycelium was extracted by water, 70% ethanol and ethyl acetate. The scavenging ability of different extracts on superoxide anion free radical, hydroxyl free radical and DPPH free radical were compared. The comprehensive antioxidant capacity of different solvent extracts was evaluated by subordinate function value, and the correlation between substance content and antioxidant capacity were analyzed. The results showed that the content of organic Ge in Ge-riched A. auricular mycelium was 542.83 μg/g. The extraction rate and substance content of different solvent extracts were different, the extraction rate of water extract and ethanol extract was higher, the polysaccharide content of water extract was the highest, and the polyphenol and flavonoid content of ethyl acetate extract was the highest. Different solvent extracts had certain scavenging ability on free radicals, and the extract concentration increased with the increase. Water extract had the strongest scavenging rate of superoxide anion free radical, when the extract concentration was 9 mg/mL, the highest clearance rate was 43.32%. 70% ethanol extract had the best scavenging effect on hydroxyl free radical and DPPH free radical. When the extract concentration was 9 mg/mL, the scavenging rate was 70.10% and 76.99%, respectively. Polysaccharides, polyphenols and flavonoids all played different roles in scavenging free radicals, and ethanol extract had the strongest comprehensive antioxidant capacity. The contents of polysaccharides, polyphenols and flavonoids were significant (P<0.05) positively correlated with DPPH free radical clearance. The content of polyphenol in 70% ethanol extract was significant (P<0.05) positively correlated with the scavenging rate of hydroxyl radical. The scavenging rate of superoxide anion radical was significantly (P<0.05) affected by polysaccharides, polyphenols and flavonoids. Ge-riched A. auricular mycelium contained a high content of organic Ge, the extract had a certain ability to clear free radicals, and had good antioxidant properties. This study would have a certain guiding significance for the development of Ge-riched A. auricular functional food.

     

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