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  • EI
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  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
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中國精品科技期刊2020
王磊,李國龍,唐志書,等. 不同生長時期酸棗果肉多糖相對分子質量分布和單糖組成及抗氧化活性研究[J]. 食品工業科技,2024,45(7):1?7. doi: 10.13386/j.issn1002-0306.2023060269.
引用本文: 王磊,李國龍,唐志書,等. 不同生長時期酸棗果肉多糖相對分子質量分布和單糖組成及抗氧化活性研究[J]. 食品工業科技,2024,45(7):1?7. doi: 10.13386/j.issn1002-0306.2023060269.
WANG Lei, LI Guolong, TANG Zhishu, et al. The Relative Molecular Mass, Monosaccharide Composition and Antioxidant Activity of Ziziphus jujuba FleshPolysaccharide at Different Stages[J]. Science and Technology of Food Industry, 2024, 45(7): 1?7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060269.
Citation: WANG Lei, LI Guolong, TANG Zhishu, et al. The Relative Molecular Mass, Monosaccharide Composition and Antioxidant Activity of Ziziphus jujuba FleshPolysaccharide at Different Stages[J]. Science and Technology of Food Industry, 2024, 45(7): 1?7. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060269.

不同生長時期酸棗果肉多糖相對分子質量分布和單糖組成及抗氧化活性研究

The Relative Molecular Mass, Monosaccharide Composition and Antioxidant Activity of Ziziphus jujuba FleshPolysaccharide at Different Stages

  • 摘要: 為了探究不同生長時期酸棗果肉中多糖含量、相對分子質量、單糖組成及抗氧化活性變化規律,以野生型和種植型酸棗為研究對象,分別采用苯酚-硫酸比色法、高效凝膠色譜法、高效離子色譜法和DPPH自由基清除實驗研究其不同生長時期的果肉多糖含量、相對分子質量、單糖組成和自由基清除能力。研究發現野生型和種植型的酸棗果肉中多糖含量均表現出先下降后上升的趨勢;兩種類型的酸棗果肉多糖的相對分子質量均隨著酸棗的發育逐漸降低;兩種類型酸棗果實發育前期果肉多糖中單糖鼠李糖含量最高可達46.14 mg/g,半乳糖次之可達33.10 mg/g;果實發育后期,兩種類型的酸棗果肉多糖中單糖含量最高的為阿拉伯糖可達60.30 mg/g,半乳糖醛酸次之可達45.02 mg/g;兩種類型酸棗果肉多糖的DPPH自由基清除能力變化趨勢與其果肉中多糖含量變化趨勢基本相同;種植型酸棗與野生型酸棗在多糖含量、多糖相對分子質量、單糖組成和自由基清除能力方面均表現為相同的趨勢,且均屬于還原糖積累類型果實。研究結果為酸棗果肉資源進一步開發與利用提供參考。

     

    Abstract: For the purpose of exploring the polysaccharide content, relative molecular mass, monosaccharide composition, as well as antioxidant activity in Ziziphus jujuba flesh polysaccharide (ZJFP) at distinct growth stages. The content, relative molecular weight, monosaccharide composition and free radical scavenging ability of ZJFP in wild-type and plant-type at different growth stages were studied by phenol-sulfuric acid assay, high performance gel chromatography, high performance ion chromatography, and DPPH free radical scavenging experiment. Moreover, the results determined that the polysaccharide content of ZJFP in both wild and plant types demonstrated a trend of first decreasing and consequently rising. Furthermore, the relative molecular mass of the two types of ZJFP gradually decreased with the development of the Ziziphus jujuba. During the initial stage of fruit development, the ZJFP exhibited the highest content of rhamnose, reaching 46.14 mg/g, followed by galactose up to 33.10 mg/g. Whereas, in the later stage of fruit development, the highest monosaccharide content in the two types of ZJFP was arabinose up to 60.30 mg/g, preceded by galacturonic acid up to 45.02 mg/g. Additionally, the trend of DPPH free radical scavenging capacity of ZJFP of the two types was identical to the trend of polysaccharide content in Ziziphus jujuba flesh. Plant-type and wild-type Ziziphus jujuba illustrated the identical trend regarding polysaccharide content, the relative molecular weight of polysaccharides, monosaccharide composition, as well as free radical scavenging ability, and both were of the type of fruit that reduces sugar accumulation. The research serve as a valuable reference for the future development and utilization of Ziziphus jujuba flesh resources.

     

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