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  • EI
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  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
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  • 中國生物醫學SinoMed
中國精品科技期刊2020
肖丹虹,李萍,鄧媛元,等. 基于響應面法優化雙酶同步酶解的全豌豆乳的穩定性及營養特性研究[J]. 食品工業科技,2024,45(8):235?246. doi: 10.13386/j.issn1002-0306.2023060114.
引用本文: 肖丹虹,李萍,鄧媛元,等. 基于響應面法優化雙酶同步酶解的全豌豆乳的穩定性及營養特性研究[J]. 食品工業科技,2024,45(8):235?246. doi: 10.13386/j.issn1002-0306.2023060114.
XIAO Danhong, LI Ping, DENG Yuanyuan, et al. Study on the Stability and Nutritional Properties of Double Enzyme Simultaneous Enzymatic Hydrolysis of Whole Pea Milk Based on Response Surface Method[J]. Science and Technology of Food Industry, 2024, 45(8): 235?246. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060114.
Citation: XIAO Danhong, LI Ping, DENG Yuanyuan, et al. Study on the Stability and Nutritional Properties of Double Enzyme Simultaneous Enzymatic Hydrolysis of Whole Pea Milk Based on Response Surface Method[J]. Science and Technology of Food Industry, 2024, 45(8): 235?246. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023060114.

基于響應面法優化雙酶同步酶解的全豌豆乳的穩定性及營養特性研究

Study on the Stability and Nutritional Properties of Double Enzyme Simultaneous Enzymatic Hydrolysis of Whole Pea Milk Based on Response Surface Method

  • 摘要: 豌豆作為一種大宗豆類,具有營養價值高及低致敏性等特點,但全豌豆乳體系易絮凝失穩,限制其在食品中的應用。本文以脫皮豌豆為原料,采用高壓均質耦合生物酶解(中溫α-淀粉酶和纖維素酶)處理,通過離心沉淀率等指標,以及Box-Behnken設計優化酶解工藝條件,揭示最優工藝下全豌豆乳的穩定性和營養特性變化規律。結果表明,相較于傳統過濾、單一酶解、雙酶分步酶解等方法,中溫α-淀粉酶和纖維素酶雙酶同步酶解制備的全豌豆乳穩定性更優,最佳的酶解工藝參數為:中溫α-淀粉酶:纖維素酶為4.5:5.5(酶活力比,U/g淀粉),添加總量為12 U/g,酶解時間為65 min,該條件下制得的全豌豆乳的離心沉淀率最低(27.70%),在不經過濾和不添加穩定劑的情況下,貯藏60 d內無明顯的沉淀分層。與傳統單體營養素復配工藝得到的豌豆乳相比,該優化工藝下制得的全豌豆乳穩定性顯著提高,淀粉的消化程度降低了12.74%,蛋白質的消化程度提高了16.41%。研究結果為濃漿類的植物乳開發提供一定的理論指導。

     

    Abstract: Peas had high nutritional values and low allergenicity as a bulk pulse. However, whole pea milk was prone to flocculation instability, which limited its application in food. In this paper, peeling peas were used as raw material and treated with high pressure homogenization coupled biological double enzymatic hydrolysis (medium temperature α-amylase and cellulase), and the stability and nutritional characteristics of whole pea milk were revealed under the optimal process through centrifugation precipitation rate and Box-Behnken design. The results showed that the stability of whole pea milk prepared by simultaneous enzymatic hydrolysis of α-amylase and cellulase was better than the counterparts processed with traditional filtering, individual enzymatic hydrolysis, and step hydrolysis of double enzymes. The optimal parameters of simultaneous enzymatic hydrolysis was as follows: Medium temperature α-amylase:cellulase was 4.5:5.5 (enzyme activity ratio, U/g starch), with a total addition of 12 U/g and 65 minutes of enzymic hydrolysis. The centrifugal precipitation rate of whole pea milk processed under the optimal conditions was the lowest (27.70%), and no significant precipitation stratification during 60 days of storage without filtration and without stabilizer. Compared with the pea milk obtained by the traditional individual nutrients formulation, the whole pea milk prepared under the optimized simultaneous enzymatic hydrolysis showed significantly improved stability. The starch digestion extent showed a reduce of 12.74%, while the protein digestion extent was increased by 16.41%. The study provides the theoretical guidance for the development of concentrated plant based milk.

     

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