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中國精品科技期刊2020
劉靜,金娜,石春芹,等. 響應面法優化豌豆蛋白植物肉配方及其體外消化分析[J]. 食品工業科技,2024,45(8):216?226. doi: 10.13386/j.issn1002-0306.2023050338.
引用本文: 劉靜,金娜,石春芹,等. 響應面法優化豌豆蛋白植物肉配方及其體外消化分析[J]. 食品工業科技,2024,45(8):216?226. doi: 10.13386/j.issn1002-0306.2023050338.
LIU Jing, JIN Na, SHI Chunqin, et al. Optimization of Pea Protein Plant Meat Formulation by Response Surface Methodology and Its in Vitro Digestion Analysis[J]. Science and Technology of Food Industry, 2024, 45(8): 216?226. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050338.
Citation: LIU Jing, JIN Na, SHI Chunqin, et al. Optimization of Pea Protein Plant Meat Formulation by Response Surface Methodology and Its in Vitro Digestion Analysis[J]. Science and Technology of Food Industry, 2024, 45(8): 216?226. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050338.

響應面法優化豌豆蛋白植物肉配方及其體外消化分析

Optimization of Pea Protein Plant Meat Formulation by Response Surface Methodology and Its in Vitro Digestion Analysis

  • 摘要: 隨著人們對健康飲食和環境保護意識的增強,植物肉產品在國內外得到迅速發展。本研究旨在探究豌豆組織蛋白與谷朊粉之比、紅薯淀粉、菜籽油、紅曲紅添加量對植物肉感官評分、質構特性咀嚼性的影響。在單因素實驗基礎上,采用Box-Behnken響應面試驗、質構分析對植物肉配方進行優化,并對最優配方進行理化指標測定和體外消化試驗。結果表明,豌豆組織蛋白與谷朊粉之比為7:3(g/g)、紅薯淀粉添加量6%、菜籽油添加量10%、紅曲紅添加量0.03%時最佳,此時得到感官綜合評分為88.31分,質構分析咀嚼性為3.131;最優配方得到的植物肉蛋白質含量為15.70%、脂肪含量為2.33%、水分含量為57.79%;經質構分析,植物肉與豬肉里脊、牛肉、雞肉除彈性外,硬度、粘結性、咀嚼性存在顯著性差異(P<0.05);體外消化分析結果顯示,植物肉的蛋白質體外消化率為80.83%,牛肉蛋白質消化率為87.50%,兩者差異性較小,說明植物肉具有較好的消化能力。以豌豆組織蛋白為原料的植物肉為進一步深入開發應用及其他相關產業提供了參考資料。

     

    Abstract: With the increasing awareness of healthy diet and environmental protection, plant-based meat products have been rapidly developed at home and abroad. The objective of this study was to examine how the ratio of pea tissue protein to gluten, along with the additions of sweet potato starch, rapeseed oil, and red yeast red, affected the sensory scores and chewiness of plant-based meat. On the basis of single factor experiments, the plant-based formula was optimized using Box Behnken response surface methodology and texture analysis, and the optimal formula was tested for physicochemical indicators and in vitro digestion tests. The results showed that the ratio of pea tissue protein to gluten was 7:3 (g/g), along with 6% sweet potato starch, 10% rapeseed oil, and 0.03% safflower red, resulted in plant-based meat with favorable sensory scores (88.31) and chewability (3.131). The protein content of the plant-based meat obtained from the optimal formula was 15.70%, the fat content was 2.33%, and the moisture content was 57.79%. According to texture analysis, there were significant differences in hardness, adhesiveness, and chewiness between plant meat and pork loin, beef, and chicken, in addition to elasticity. The results of in vitro digestion analysis showed that the protein digestibility of plant meat was 80.83%, while that of beef was 87.50%. The difference between the two was small, indicating that plant meat had good digestion ability. The plant meat made from pea tissue protein provides reference materials for further development, application, and other related industries.

     

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